Example of widefield particle extinction analysis using Extinction Suite v4.0.2

Unzip Data.zip
Data.zip contains 1 folder: "Data", which should be treated as the base folder for this analysis.
"Data" contains 1 folder: "ProcessedImage".
"ProcessedImages" contains 4 developed extinction images: "Extinction_600.tif", "Extinction_550.tif", "Extinction_500.tif", "Extinction_450.tif".

The data in this example analysis was taken in the synchronously triggered stage-camera format using single recording over all color channels and using the shifted reference method, so that there is a single saved signal file, and a single saved background (dark sensor) file. The recording settings are 128 images per signal position and 128 per reference position with 10 repetitions, yielding \(128\times2\times10=2560\) total brightfield images per color channel. The background image assumes a single exposure time in brightfield for all color channels, and therefore contains only 2560 images (i.e. NOT 4\(\times\)2560 images) of the dark sensor for this single exposure time.

1. Run ExtinctionSuite v4.0.2

2. Mode Selection

3. Module Selection

4. Choose Base Folder

5. Analysis Options

Analysis options panel
          radii panel
Choose main image

6. Reference shift confirmation

Panel of detected reference

7. ROI Format

ROI Format panel
Adjust ROI

8. Extinction Noise Tolerance

Noise tolerance introduction
Example of workspace at noise
          tolerance dialogue
Example workspace at noise
              tolerance dialogue using preview
Noise Tolerance Value

9. Image Registration

Image Registration Panel

10. Calibration of the cross-section acceptance range

Calibration histogram and dialogue
Redisplayed histogram
Histogram exploration dialogue
          in histogram
CS calibration values
Operation Complete prompt

11. Results

Contents of data folder
Contents of results folder
          for 450nm channel Histogram
          for 500nm channel
          for 550nm channel Histogram
          for 600nm channel

Created by Lukas Payne, 27/10/2019.