Analysis path: Extinction BG subtraction type: Shifted Sample Proximity restriction= 2*Ri Total # brightfield images taken per channel: 2560,2560,2560,2560 # BG datapoints taken= 1000 Center wavelength of spectral ranges: 600,550,500,450 Ranks of spectral ranges: 1,1,1,1 Numerical aperture= 0.95 Magnification= 40 Pixel size= 6.5 Full well capacity= 30000 Used Gaussian Fit for Particle Center Coordinates? 1 Length scale calibration= 162.5 nm/pixel Measurement radius Ri for each channel (pixels): 5.8300, 5.3441, 4.8583, 4.3725 Outer radius for each channel (pixels): 11.6599, 10.6883, 9.7166, 8.7449 Reference shift in x per color channel -12.7273, -12.8846, -12.8462, -12.5000 Reference shift in y per color channel 0.6364, 0.1538, 0.6154, 0 X Coordinate of Selection Rectangle = 15 Y Coordinate of Selection Rectangle = 15 Width of Selection Rectangle = 1252 Height of Selection Rectangle = 1253 Drift detected in x direction (pixels): 0, -0.1538, -0.3333, -0.9688 Drift detected in y direction (pixels): 0, -0.0385, -0.2222, -0.4062 Successfully Identified Maxima Successfully completed promixity filtering Beginning background point identification... Background identification successful Lower limit of acceptance range: 4000 Upper limit of acceptance range: 12000