uFLIM Software

The uFLIM program was developed by Francesco Masia and Wolfgang Langbein to analyze FLIM-FRET data,  specifically to retrieve fluorescence components with different lifetimes for unmixing and to determine FRET rate distribution parameters in a FLIM-FRET experiment. The method is based on non-negative matrix factorisation.

The details of the implemented algorithms are described in the following papers.

Please acknowledge the usage of the uFLIM software and cite our related publications.

Installing & Running the Hyperspectral Imaging Analysis (HIA) software


Created by Francesco Masia, 10/11/2021, transferred 11/11/2021 WL, Updated 01/04/2023 WL