Coherent Anti-Stokes Raman Scattering microscopy for non-invasive imaging of living cells

Optical microscopy is an indispensable tool for cell biology. Different microscopy methods are currently available and continuous effort is devoted to develop new techniques with improved sensitivity, selectivity and/or spatial resolution. An important issue in recent microscopy is the ability to perform non-invasive studies, i.e. avoiding the need for fluorescent probes that are prone to photo bleaching and can perturb the cell functions. The research plan is to develop a new generation of laser-scanning multiphoton microscope based on Coherent Anti-Stokes Raman Scattering (CARS, see figure below) with improved sensitivity and chemical specificity for real-time studies on living cells. Such an instrument will allow non-invasive microscopic examination of cells and subcellular structures under physiological conditions without the need to stain or express tagged proteins.
 Coherent Anti-Stokes Raman Scattering
Paola Borri aligning the microscope setup (05/2007) Israel Rocha-Mendoza, Wolfgang Langbein and Paola Borri and the CARS Microscope setup (05/2007)